- #Microsynth single tube sequencing serial
- #Microsynth single tube sequencing full
- #Microsynth single tube sequencing code
#Microsynth single tube sequencing code
The analysis code can be found at GitHub ( ). This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All scRNA-seq and RNA-seq data in this study are deposited in NCBI Gene Expression Omnibus under accession number GSE142645. Received: OctoAccepted: MaPublished: May 2, 2023Ĭopyright: © 2023 Wu et al. PLoS Biol 21(5):Īcademic Editor: Connie J.
(2023) Single-cell transcriptome analyses reveal critical roles of RNA splicing during leukemia progression.
#Microsynth single tube sequencing full
Our study emphasized the critical roles of RNA splicing in the full process of leukemogenesis.Ĭitation: Wu B, Chen X, Pan X, Deng X, Li S, Wang Z, et al. While the exon skipping product Tmem134β promoted the cell cycle, full-length Tmem134α delayed tumorigenesis. Exon 4 skipping of Tmem134 was identified in these cells. Among them, the Hsp90aa1 hi subpopulation was conserved in both human and mouse AML and associated with poor prognosis. In the late stage, PLCs acquired explosive heterogeneity through RNA alternative splicing. Furthermore, we dissected the detailed gene network of a tipping point for hematopoietic stem and progenitor cells (HSPCs) to generate initiating PLCs, which was characterized by dramatically increased splicing factors and unusual RNA velocity. Among them were splicing factors, which showed stage-specific prognosis for AML patients. We found that the Myc targets were gradually up-regulated along the trajectory.
#Microsynth single tube sequencing serial
Here, we performed serial single-cell transcriptome analyses of preleukemic and leukemic cells (PLCs) and constructed the cellular and molecular transformation trajectory in a Myc-driven acute myeloid leukemia (AML) model in mice, which represented the transformation course in patients. One interesting thing is that positionhigh has produced a short video showing how they see the future development panning out.Leukemogenesis is proposed to be a multistep process by which normal hematopoietic stem and progenitor cells are transformed into full-blown leukemic cells, the details of which are not fully understood. All the links you need are provided by the developer “positionhigh” on their page. However, to purchase it, you have to navigate the mystical portals of PCBWay and source of your own parts, including the 3D printing of the case. It also has an arpeggiator.Īll-in-all, you get a pretty groovy synth and groove-box machine. You can stack up to 7 notes into chords on a single track. The sequencer has 8-tracks, 16 step patterns, pattern chaining and a ridiculous amount of steps. You also find the firmware from the Mutable Instruments Braids oscillator with up to 8-voice poly and added envelopes and filters. On the sampling side, you can load, edit and play multi-sampled instruments. The MicroSynth adds two virtual analogue monophonic synthesizers with a single oscillator, multiple waveforms, pulse width modulation, a filter and noise generation.
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